The movie centers around Jamie, a butch lesbian actress facing challenges in her career, who lands a role portraying a man in a film. The central storyline is a romantic comedy involving Jamie’s male persona, “Male Jamie,” and Jill, a straight woman working on the film set. The film also explores several subplots, including the life of Jamie’s bisexual roommate Lola and her feline co-star Howard, Lola’s tough butch German girlfriend Andi, and Jamie’s gay Asian friend David.